Journal: EBioMedicine

Article Title: Renal interstitial fibroblasts coproduce erythropoietin and renin under anaemic conditions

doi: 10.1016/j.ebiom.2021.103209

Figure Lengend Snippet: Renal interstitial cells express renin in kidneys of CKD patients, and a schematic diagram of Epo and renin production in kidneys. (a) Representative images of ISH of REN (brown) in kidney sections of patients suffering from chronic tubulointerstitial nephritis. Arrowheads and dotted circles are interstitial fibroblasts positive for REN mRNA expression and glomeruli, respectively. Red and blue arrows indicate JG cells that are positive and negative for REN mRNA expression, respectively. The scale bar is 100 μm. (b) A schematic diagram of Epo and renin production in the kidney under normal, anaemic, and injured conditions. Renal interstitial fibroblasts (yellow) maintain the systemic oxygen supply under anaemic conditions through inducing Epo and renin production in response to hypoxia and hypotension, respectively. In injured kidneys, most interstitial fibroblasts are transformed into myofibroblasts (orange), which lose the ability to produce Epo and enhance renin production.

Article Snippet: Formalin-fixed paraffin-embedded kidney sections from patients suffering from chronic tubulointerstitial nephritis were purchased from OriGene (CS700464 and CS705284 for Patient 1 and Patient 2, respectively).

Techniques: Expressing, Transformation Assay

Journal: Nature methods

Article Title: Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples

doi: 10.1038/nmeth.2483

Figure Lengend Snippet: Shown are key metrics for RNase-H (orange), Ribo-Zero (pink) and total (black) libraries from pancreas and FFPE kidney RNA. ( a ) Percent of rRNA mapping reads; ( b ) Proportion of reads mapping to exons (solid), introns (hatched), and intergenic (white) regions; ( c ) mean coefficient of variation ( y axis) for the top 1,000 expressed transcripts in each library ( x axis); ( d ) Pearson correlation coefficient between each library and a control Total library.

Article Snippet: For human kidney FFPE tissue sections (Cybrdi), we isolated total RNA with the MasterPure RNA Purification Kit (Epicentre) following the manufacturer’s instructions with these modifications.

Techniques: Control

Journal: Cancer Science

Article Title: Radioimmunotherapy of human synovial sarcoma using a monoclonal antibody against FZD10

doi: 10.1111/j.1349-7006.2007.00701.x

Figure Lengend Snippet: Immunohistochemical staining analyses of synovial sarcoma and normal human tissue sections using MAb 92‐13; (a,b) synovial sarcoma tissue sections (c) colon, (d) heart, (e) lung, (f) liver, (g) kidney and (h) placenta. Original magnification ×100. Bar, 50 µm.

Article Snippet: For immunohistochemical staining analysis, 4‐µm sections of paraffin‐embedded normal adult human tissues (lung, heart, liver, kidney, colon and placenta) (BioChain, Hayward, CA, USA) and surgical synovial sarcoma specimens were deparaffinized and processed for antigen retrieval in Target Retrieval Solution (pH 9) (DAKO Cytomation, Carpinteria, CA, USA) at 125°C for 30 s. After treatment with peroxidase blocking reagent (DAKO Cytomation) for 10 min, followed by treatment with protein blocking serum‐free (DAKO Cytomation) for 30 min, slides were incubated with 10 µg/mL MAb 92‐13.

Techniques: Immunohistochemical staining, Staining